h2b mruby Search Results


a549 expressing h2b mruby  (ATCC)
99
ATCC a549 expressing h2b mruby
A. Schematic of the screen. <t>A549</t> cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).
A549 Expressing H2b Mruby, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a549 expressing h2b mruby/product/ATCC
Average 99 stars, based on 1 article reviews
a549 expressing h2b mruby - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
plenti hygro h2b mruby backbone  (Addgene inc)
93
Addgene inc plenti hygro h2b mruby backbone
A. Schematic of the screen. <t>A549</t> cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).
Plenti Hygro H2b Mruby Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenti hygro h2b mruby backbone/product/Addgene inc
Average 93 stars, based on 1 article reviews
plenti hygro h2b mruby backbone - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
aav9-cag-dio-chronosm140e-st-p2a-h2b-mruby  (Addgene inc)
90
Addgene inc aav9-cag-dio-chronosm140e-st-p2a-h2b-mruby
A. Schematic of the screen. <t>A549</t> cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).
Aav9 Cag Dio Chronosm140e St P2a H2b Mruby, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aav9-cag-dio-chronosm140e-st-p2a-h2b-mruby/product/Addgene inc
Average 90 stars, based on 1 article reviews
aav9-cag-dio-chronosm140e-st-p2a-h2b-mruby - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
somatic opsin st chrome  (Addgene inc)
92
Addgene inc somatic opsin st chrome
A. Schematic of the screen. <t>A549</t> cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).
Somatic Opsin St Chrome, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/somatic opsin st chrome/product/Addgene inc
Average 92 stars, based on 1 article reviews
somatic opsin st chrome - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
paav cag h2bmruby2flag plasmid 116872  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
mruby h2b 6 plasmid 55866  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
mruby2 h2b 6 plasmid 55897  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
mruby2 h2b c 10 plasmid 55898  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
aav syn chr2 kv p2a h2b mruby2 plasmid 89570  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
pcs2 h2b mruby2 plasmid 99270  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
pkancmv mruby3 10aa h2b plasmid 74258  (Addgene inc)
N/A
   Buy from Supplier
pcag h2b mruby3 ires egtacr1 st plasmid 108960  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier

Image Search Results


A. Schematic of the screen. A549 cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).

Journal: bioRxiv

Article Title: Drug repurposing screen identifies masitinib as a 3CLpro inhibitor that blocks replication of SARS-CoV-2 in vitro

doi: 10.1101/2020.08.31.274639

Figure Lengend Snippet: A. Schematic of the screen. A549 cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).

Article Snippet: A549 expressing H2B-mRuby were generated by first infecting A549 cells (ATCC CCL-185) with a lentivirus (carrying H2B-mRuby), and FACS-sorting mRuby+ cells.

Techniques: Expressing, Infection, Incubation, Staining

Of the 26 drugs that inhibited OC43 and tested against SARS-CoV-2, 20 inhibited SARS-CoV-2 replication in a dose-dependent manner, showing good concordance between OC43 and SARS-CoV-2 inhibition. A. A549 cells over-expressing ACE2 were pre-treated with indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and incubated for 2 days. Cells were stained for the presence of the spike protein and the % of infected cells was analyzed. Most of the drugs effective against OC43 showed similar effectivity against SARS-CoV-2, n=3. Individual measurements are shown as semi-transparent circles (note that some circles overlap). B. Effect of selected drugs on SARS-CoV-2 progeny production. Cells were treated with 10 μM of the indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and cell supernatant were collected for titration 2 days later, n = 3. Individual measurements are shown as semi-transparent circles. All drugs showed a statistically significant (p-values<0.001, one-tailed t-test, FDR-corrected) reduction in viral titers.

Journal: bioRxiv

Article Title: Drug repurposing screen identifies masitinib as a 3CLpro inhibitor that blocks replication of SARS-CoV-2 in vitro

doi: 10.1101/2020.08.31.274639

Figure Lengend Snippet: Of the 26 drugs that inhibited OC43 and tested against SARS-CoV-2, 20 inhibited SARS-CoV-2 replication in a dose-dependent manner, showing good concordance between OC43 and SARS-CoV-2 inhibition. A. A549 cells over-expressing ACE2 were pre-treated with indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and incubated for 2 days. Cells were stained for the presence of the spike protein and the % of infected cells was analyzed. Most of the drugs effective against OC43 showed similar effectivity against SARS-CoV-2, n=3. Individual measurements are shown as semi-transparent circles (note that some circles overlap). B. Effect of selected drugs on SARS-CoV-2 progeny production. Cells were treated with 10 μM of the indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and cell supernatant were collected for titration 2 days later, n = 3. Individual measurements are shown as semi-transparent circles. All drugs showed a statistically significant (p-values<0.001, one-tailed t-test, FDR-corrected) reduction in viral titers.

Article Snippet: A549 expressing H2B-mRuby were generated by first infecting A549 cells (ATCC CCL-185) with a lentivirus (carrying H2B-mRuby), and FACS-sorting mRuby+ cells.

Techniques: Inhibition, Expressing, Infection, Incubation, Staining, Titration, One-tailed Test